Weekly Update 3 - Genetics
This week: Replication, Transcription, Translation, with a little bit of regulation.
This week we will be looking at bacterial genetics by focusing on the three core genetic processes: Replication, Transcription and Translation. While we do this, we will be looking at some genetic regulation as well.
Suggested Readings:
Learning Objectives
At this point, all students should be able to provide a solid description of Replication, Transcription and Translation. Therefore, we will not concern ourselves with the basics of these processes, but instead focus on how Bacteria differ from Eukarya in these three processes. As we move though this week, we will also begin our discussion on genetic regulations.
This week we will be looking at bacterial genetics by focusing on the three core genetic processes: Replication, Transcription and Translation. While we do this, we will be looking at some genetic regulation as well.
Suggested Readings:
- Slonczewski and Foster textbook:
- Chapter 7: Genome and Chromosome
- Chapter 8: Transcription, Translation and Bioinformatics
- The following Wikipedia articles have good information on some specific topics of interest with bacterial genetics. They also provide good references for you to use in your own studies:
- Nucleoid - This is a good article because it makes a distinction between a chromosome and a genophore (bacterial DNA).
- Sigma Factor - The textbook give a more complete discussion on sigma factors, but this article has a nice list of sigma factors.
- Bioinformatics Organization FAQ
Learning Objectives
At this point, all students should be able to provide a solid description of Replication, Transcription and Translation. Therefore, we will not concern ourselves with the basics of these processes, but instead focus on how Bacteria differ from Eukarya in these three processes. As we move though this week, we will also begin our discussion on genetic regulations.
- Be able to discuss the concept of a genome, and what constitutes a bacterial genome.
- Be able to discuss supercoiling of DNA and the use of topoisomerases.
- Be able to discuss bacterial DNA replication (you may need to go back to the chapter on prokaryotic cells).
- Be able to discuss plasmid DNA and the importance of Bacteriophages.
- Be able to discuss how DNA analysis is done, and why.
- Be able to discuss how researchers analyze the whole genome, and why it is important.
- Be able to discuss the significance of the sigma factor to RNA Polymerase and bacterial transcription.
- Be able to discuss the bacterial promoter and how it is different to the eukaryote promoter.
- Be able to discuss Transcription termination in terms of either Rho-dependent or Rho-independent mechanisms.
- Be able to discuss all of the different types of RNA.
- Be able to discuss bacterial translation, including the phenomena of polyribosomes and the size difference between bacterial and eukaryote ribosomes.
- Be able to discuss post-translational modification of proteins.
- Be able to discuss the bacterial secretion mechanisms.
- Be able to describe open reading frames, paralogs, orthologs and DNA alignments.
- Be able to discuss the Lac operon and its regulation.
No comments:
Post a Comment